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PHOTO-INDUCED
OXIDATIVE PHENOMENA AND MEMBRANE STABILITY
My
research activities are developed in the Biophotonic team
of the Laboratory Jean Perrin (CNRS UMR 8237) at Pierre
and Marie Curie University. These activities are
interested, under physical approaches, in the optical
properties of biological systems. Our team works not only
on the study and the characterization of biologically
photo-active compounds but also on the apparatuses and
methods by which such studies become possible.
My
personal interests relate to the comprehension of the
cellular behaviour of the molecules in relation with:
their behaviour in the intracellular
traffic, their physical and
physicochemical properties, the properties of biological
membranes and
their possible photo-modulation.
Key words - Membrane-molecules interactions. Amphiphilic
dyes transport through membranes. Photosensitized damage
in biophysical systems. Photodynamic treatment of cancer
cells.
2003-2005
Study of axonal transport and mechanical
properties of the astrocytes by new physical techniques :
Post-doc in the LNPC (EA 3817, Université de Paris 5)
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Confocal microscopy,
AFM, Quantums
dots
in biology, Surface coating and
treatment, Gial and PC12 Cell's
Culture and differenciation...
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1999-2003
Biophysical studies of the
interaction of a porphyrin with lipoproteins and models
of membrane: consequences on cellular incorporation and
targeting: Thesis and DEA supervised by D. Brault, (UMR
CNRS 8646 - INSERM U 201, laboratory of biophysics,
National Museum of Natural History, Paris).
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Optical
spectroscopies, fast kinetics, Fluorescence
microscopy, Analysis and data-processing
simulation of dynamic
systems in solution, with MathCad, Biophysical and
biochemical methods (ligand-receptor
interaction, Scatchard, kinetics,
enzymology, membrane permeability,
solubility…).
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[Lien] [Full text]
2001-2002
Development
and evaluation of detection methods of a photosensitizing
drug (in vitro and in vivo approaches) :
industrial valuation (CNRS/Negma-LERADS).
1998-1999
Study
of a parietal glycoprotein of Phytophthora parasitica
var. nicotianae, a Tabacco's parasite : DESU (12
months) in the team of M.-T. Esquerre, (UMR CNRS - UPS
5546, Toulouse).
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Molecular
biology methods: Extraction (ARN,
ADN, Proteins), Electrophoresis, Northern
Blot, Southern Blot, PCR... Biochemistry
methods:
Electrophoresis, Chromatographies of
proteins...
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As
associate professor
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Educational
coordinator of the first year of Master
of Physics : Click Here.
Coordinator of the
second year of Licence of Physics and
interfaces, 2005-2010 : Click Here.
Biology for
physicists lessons, M2 Biophysics.
Biological optics,
M2 Optics, matter and plasma.
Lectures and TD of
Applied statistical physics, M1 Applied
Pysics.
Master classes (project
of first year of master) in M1 physics.
Undergraduate
teaching:
In L3 PSVP: TD of
LP347, LP343, lectures LP390 "Biological
and medical Imaging", lectures LP392 "Bioenergetics:
thermodynamical aspects of Life" for physicists.
In L2 Physics and
interfaces: Thermodynamics lectures.
Past
teaching:
Doctoral lectures
on optical spectroscopies.
Lectures for Master's
degree students (M2 of Molecular and
Cellular Biology). Photo-destabilisation
of membranes and Photo-Chemical
internalisation.
Biology for
Physicists lessons, M2 Optics, matter
and plasma.
Biophysics lectures, M1
Applied physics.
For the first year
students in the pluridisciplinary program
("Science Po"/UPMC): LS120
"Thermodynamics".
TD of
thermodynamics and biophysical
appications in L2 PI.
LV303 "Biochemistry" (lectures
and TD)
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